Metabolite Detection Assays

Our metabolite detection assays utilize state-of-the-art LC-MS/MS technology to quantify endogenous small molecules in complex biological matrices with exceptional sensitivity and specificity. These assays enable precise measurement of metabolic pathways, providing critical insights into disease mechanisms, therapeutic efficacy, and biomarker discovery.

Technology Specifications

Detection Limit

Sub-femtomolar (fM) sensitivity for most metabolites

Dynamic Range

8-10 orders of magnitude for comprehensive quantification

Sample Throughput

Up to 500 samples per day with automated processing

Metabolite Coverage

Over 2,000 annotated metabolites across major pathways

Assay Workflow

  1. Sample Preparation: Optimized extraction protocols for blood, tissue, urine, and cell culture samples with minimal matrix interference
  2. Chromatographic Separation: Ultra-high-performance liquid chromatography (UHPLC) with tailored column chemistry for metabolite class separation
  3. Mass Spectrometry: Triple quadrupole and high-resolution mass spectrometry for accurate mass measurement and structural confirmation
  4. Data Analysis: Custom bioinformatics pipeline for peak detection, quantification, and pathway enrichment analysis
  5. Validation: Rigorous quality control with internal standards and replicate analysis to ensure precision and accuracy

Key Applications

Disease Biomarker Discovery

Identification of metabolite signatures associated with disease progression and therapeutic response in oncology, neurology, and infectious diseases.

Drug Metabolism Studies

Quantification of drug metabolites to assess pharmacokinetics, toxicity profiles, and metabolic pathway engagement.

Metabolic Pathway Analysis

Systems-level mapping of metabolic networks to understand cellular responses to environmental cues and genetic perturbations.

Therapeutic Efficacy Monitoring

Tracking metabolic changes in response to treatments to develop personalized medicine strategies and optimize therapeutic outcomes.

Assay Performance Metrics

  • Intra-assay precision: < 5% coefficient of variation (CV)
  • Inter-assay precision: < 8% coefficient of variation (CV)
  • Recovery rates: 85-115% across all sample types
  • Stability: > 72 hours at 4°C and > 6 months at -80°C

Custom Metabolite Assay Development

We offer tailored metabolite profiling solutions for academic and industry partners. Our team can develop custom assays for specific metabolite targets or pathway-focused panels.

Request Custom Assay