Molecular Binding Assays

Quantifying biomolecular interactions with high precision for drug discovery and target validation

Our molecular binding assays provide quantitative measurements of interactions between small molecules, peptides, and proteins—critical for identifying promising drug candidates and understanding their mechanism of action. These assays enable precise determination of binding affinity (Kd), specificity, and kinetic parameters (kon/koff) to support rational drug design and target validation.

Assay Technologies

Fluorescence-Based Assays

Homogeneous and heterogeneous fluorescence assays including FRET, TR-FRET, and fluorescence polarization techniques for rapid screening of compound libraries.

Key Applications: High-throughput screening, fragment-based drug discovery, competitive binding studies

Surface Plasmon Resonance (SPR)

Label-free technology for real-time monitoring of biomolecular interactions, providing kinetic and affinity data without requiring fluorescent tags.

Key Applications: Kinetic parameter determination, epitope mapping, concentration analysis

Isothermal Titration Calorimetry (ITC)

Direct measurement of binding thermodynamics (ΔH, ΔS, ΔG) to characterize the energy landscape of molecular interactions.

Key Applications: Mechanistic studies, thermodynamic profiling, stoichiometry determination

Performance Characteristics

Affinity Range: pM to mM
Throughput: 96/384/1536-well formats
Sample Consumption: µL to mL scale
Instrumentation: Biacore SPR, PHERAstar FS, MicroCal ITC200
Automation: Liquid handling integration
Data Output: Kd, kon, koff, ΔH, ΔS values

Research Applications

  • Screening of small molecule libraries against viral targets including DENV NS3 protease and influenza neuraminidase
  • Characterization of antibody-antigen interactions for therapeutic antibody development
  • Evaluation of peptide binding to cancer oncoproteins and signaling receptors
  • Determination of compound specificity across related protein families to minimize off-target effects
  • Analysis of protein-drug binding in the presence of resistance-conferring mutations
  • Validation of computationally predicted binding interactions from AlphaFold3 models

Need Custom Binding Assay Development?

We offer collaborative assay development services tailored to specific therapeutic targets and research questions.

Inquire About Collaboration