Cell Signaling Assays

Advanced systems for decoding cellular communication networks and their role in disease pathways

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Reporter Gene Assays

Dual-luciferase and GFP-based reporter systems to quantify pathway activation downstream of key receptors and transcription factors with high precision.

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Phosphoprotein Analysis

High-sensitivity ELISA and Western blot protocols to measure protein phosphorylation events at critical signaling nodes with subcellular resolution.

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Dose-Response Profiling

Automated liquid handling and detection to generate precise EC50/IC50 curves for pathway modulation with enhanced data reproducibility.

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Kinase Activity Assays

Target-specific kinase assays to identify direct modulators of key signaling enzymes in disease pathways with high-throughput capabilities.

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Key Pathways Under Investigation

Our research focuses on critical signaling networks involved in disease progression and therapeutic intervention

NF-κB Signaling
JAK-STAT Pathway
MAPK/ERK Cascade
PI3K/Akt/mTOR Axis
Type I Interferon Response
Apoptosis Signaling

Primary Applications

Identifying pathway-selective modulators for targeted therapeutics development
Mechanism of action studies to understand compound interactions
Structure-activity relationship (SAR) development for optimized efficacy
Profiling compound selectivity across related signaling networks
Disease modeling through pathway dysregulation analysis
Biomarker identification for drug response prediction